![]() ![]() ![]() Transcription: " Yamiyo no Ressha" ( Japanese: 闇夜の列車)Īt a Marleyan military meeting, Zeke proposes to use his last year of life to attack Paradis Island and retake the Founding Titan. However, after witnessing the developed anti-Titan weaponry, Marley realizes the need to resume the operation to retrieve the Founding Titan. ![]() Zeke, noting that the cause of this war was the Warriors’ defeat, transforms into the Beast Titan and annihilates the Mid-East Allied Fleet, thus ending the four-year war, with Marley victorious. Reiner's Armored Titan, aided by the Jaw Titan, successfully pulverizes the fort's defenses, including another armored train. From an airship above, Eldians are transformed into Pure Titans by Zeke using his scream and are dropped onto the fort, destroying much of its structure. This allows Pieck's Cart Titan and Galliard's Jaw Titan to attack the fort. ![]() Gabi manages to approach the train and disable it with hand grenades. Colt proposes to Commander Theo Magath to send in the Warrior Titans, but he refuses due to the Allied Forces' armored train possessing a deadly anti-Titan cannon. In the final battle at the Allied Forces' base, Fort Slava, Marley sent Eldian soldiers including Warrior Candidates (candidates to inherit the power of the Nine Titans) Falco Grice, his older brother Colt, Reiner's cousin Gabi Braun, Udo and Zofia. As a result, the operation to reclaim the Founding Titan was put on hold. Transcription: " Umi no Mukōgawa" ( Japanese: 海の向こう側)įour years after the Warrior Unit's defeat on Paradis Island, Marley is at war against the Mid-East Allied Forces. For the first special of Part 3, the ending theme is " Under the Tree" performed by SiM. "A Child of Evil") performed by Ai Higuchi. For Part 2, the opening theme is " The Rumbling" performed by SiM, and the ending theme is "Akuma no Ko" ( 悪魔の子, lit. "My War") performed by Shinsei Kamattechan, and the ending theme is "Shōgeki" ( 衝撃, lit. For Part 1, the opening theme is " Boku no Sensō" ( 僕の戦争, lit. The score is directed by Masafumi Mima and composed by Hiroyuki Sawano and Kohta Yamamoto. JST while the second half will debut in fall 2023. A third and final part, subtitled The Final Chapters, will air in two halves the first half, a one-hour special, premiered on March 4, 2023, at 12:25 a.m. A second part aired on NHK General TV from January 10 to April 4, 2022, at 12:05 a.m. In Southeast Asia, the subbed series was released on iQiyi. In the United States, Adult Swim's Toonami programming block began airing Funimation's English dub on January 10, 2021, at 12:30 a.m. The first part of the season aired on NHK General TV from December 7, 2020, to March 29, 2021, at 12:10 a.m. In the third and final part of the season, the Alliance led by members of the Survey Corps and Warrior Unit head to Marley in order to stop Eren's Founding Titan and end the Rumbling. In the second part of the season, aware of the global anti-Eldian sentiment resulting from Marleyan propaganda, Eren Jaeger preemptively targets the world outside of Paradis with the Rumbling, unleashing millions of Colossal-like Wall Titans in a widespread effort to kill all life beyond the island. As Marley and the Paradis forces go to war in Marley and the Shiganshina District, both sides sustain a heavy death toll while Gabi and Falco are forced to confront their internal tensions about the supposed "devils" of Paradis. While Marley plans to invade Paradis to strengthen their weakening military and retrieve the Founding Titan, the Survey Corps lay an attack in their homeland. The season introduces Gabi Braun and Falco Grice, young Eldian Warrior candidates seeking to inherit Reiner's Armored Titan four years after the failed mission to reclaim the Founding Titan. The season covers the " Marley" (chapters 91–106) and " War for Paradis" (chapters 107–139) arcs from the original manga by Hajime Isayama. Scriptwriter Hiroshi Seko fully took over the series composition from Yasuko Kobayashi, and Tomohiro Kishi replaced Kyōji Asano as character designer due to the series switching production studios. The fourth and final season of the Attack on Titan anime television series, subtitled Attack on Titan: The Final Season, was produced by MAPPA, chief directed by Jun Shishido, and directed by Yuichiro Hayashi, replacing Tetsurō Araki and Masashi Koizuka, respectively. ![]()
0 Comments
9/18/2023 0 Comments Western blot results ncbi![]() Protein transfer with subsequent immunodetection has found wide application in the fields of life sciences and biochemistry. The utility of the high resolving power of SDS PAGE ( 5) was limited in purpose, owing to the fact that the separated proteins in the gel matrix were difficult to access with molecular probes, until the advent of protein blotting. (C) Primary antibody binding to a specific band on the blot (D) Secondary antibody conjugated to an enzyme (alkaline phosphatase or horse radish peroxidase) binding to primary antibody (E) color development of specific band (Reproduced from Ref 10 with permission from Elsevier). The bands shown are hypothetical (B) Exact replica of SDS PAGE gel obtained as a blot following Western transfer. (A) Unstained SDS PAGE gel prior to Western blot. Schematic representation of Western blotting and detection procedure Dot blotting refers to the analysis of proteins applied directly to the membrane rather than after transfer from a gel. The subsequent employment of antibody probes directed against the membrane bound proteins (immunoblotting) has revolutionized the field of immunology ( Fig. The blotted proteins form an exact replica of the gel and have proved to be the starting step for a variety of experiments. The term ‘western blotting’ was coined to describe ( 5) this procedure to retain the ‘geographic’ naming tradition initiated by Southern’s paper ( 3) ( see Chapter 3). Protein blotting evolved from DNA (Southern) blotting ( 3) ( see Chapter 1) and RNA (Northern) blotting ( 4). Nucleic acids are routinely transferred from agarose gels, to a membrane support, through capillary action (Southern blotting). Proteins that are resolved on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) gels are typically transferred to adsorbent membrane supports under the influence of an electric current in a procedure that is known as western blotting (WB) or protein blotting ( 1, 2) ( see Chapters 2 and 3). The transfer of proteins or nucleic acids to microporous membranes is referred to as “blotting” and this term encompasses both “spotting” (manual sample deposition) and transfer from planar gels. ![]() 9/18/2023 0 Comments Clix car safe dog harness![]() Sizing Guide: Extra Small To fit chest size 35-65cm Small To fit chest size 55cm. Connect to the seatbelt receiver or slide the seatbelt through the harness. Pros & Cons Criss cross design that provides secure fitting for your dog Comes in 4 different sizes Made from safety standard approved seat belt material. The CLIX CarSafe Harness keeps your dog comfortable and secure while travelling in the car. With the option to plug the harness straight into the seatbelt receiver, or slide the seatbelt through the harness, the Clix CarSafe is designed for ultimate safety and comfort for all. All styles connect directly to your car seat belt system to create a safe dog car seat. Thanks To The Double-Sided Adjustable Buckles The Harness Clips Together Without The Need To Manipulate The Dog'S Legs And It Can Be Securely Attached By Either Plugging Straight Into The Seat-Belt Socket Or By Sliding The Seat-Belt Through The Harness. Designed by Dr Roger Mugford, the CLIX CarSafe harness combines safety with comfort for your dog in the car and whilst walking. The CarSafe dog car harness range is designed to keep your dog safe. 4. Made From Approved Safety-Standard Seat-Belt Material, The Clix Carsafe Harness Is Designed In A Continuous Loop, To Ensure That In The Unfortunate Event Of An Accident, Maximum Strength Is Retained. ![]() The Unique 'X-Cross' Design Provides An Extremely Comfortable And Ergonomic Fit, Whilst Ensuring The Harness Remains In The Optimum Position, Even Whilst Moving. The CLIX Car Safe harness has been developed to provide safety and comfort for your dog, both in the car and whilst walking. The Clix Carsafe Harness Has Been Developed To Provide Safety And Comfort For Your Dog, Both In The Car And Whilst Walking.įeatures: Soft Neoprene Padding Covers All Points Of Contact To Ensure Maximum Protection And Comfort, With Particular Focus On The Central Chest Protector. Company of Animals Clix Car Safe Harness is a secure and certified safe dog harness that will keep your pup safe when in the car. ![]() 9/18/2023 0 Comments Degenerative myopia retina![]() ![]() This helps reduce leaking of the blood vessel and oedema (fluid build up which causes swelling to the retina). Myopic choroidal neovascularisation (growth of new blood vessels) is treated with anti-VEGF injections, the anti-VEGF injection reduces or stops the growth of new blood vessels.If a retinal tear is present they can use laser (retinopexy) or freezing (cryotherapy or cryopexy) to seal the tear and stop a retinal detachment from occurring. They are monitored to ensure further retinal complications do not occur. Retinal atrophy, lattice degeneration and lacquer cracks are not usually treated.The scarring causes the patient to experience a permanent blank spot in their central vision. A circular spot of scarring is left behind on the macula. ğoster Fuchs spot is when the new blood vessels and leakage in the central vision has dissipated.If you notice any of this you must see your optician or an ophthalmologist at your local eye hospital immediately. Patients may also experience grey or blank spots in their central vision. Wet Myopic macular degeneration occurs suddenly and will affect your colour vision, ability to see detail, cause straight edges to appear very wavy, cause difficulty in recognising faces and reading.The leakage of fluid can cause damage and swelling (oedema), neovascularisation can occur anywhere on the retina including your central vision, the macula (this is called wet myopic macular degeneration). As the new blood vessels are very weak they can leak easily. Ĝhoroidal Neovascularisation (CNV), are new blood vessels that have grown from the Choroid (layer under the retina) and passed between any breaks or tears in the retina.Untreated Glaucoma causes a patient to lose their peripheral vision over time. Patients with degenerative myopia should undergo intensive visual fields yearly. This causes a build up of fluid, as the drainage angle is impaired, the eye pressure begins to rise and causes damage to the optic nerves. Glaucoma the drainage angle of eye can be affected with degenerative myopia.Patients with degenerative myopia should undergo a dilated fundus (retina, nerve and macula) check yearly. If you experience this you must get to your local hospital eye service immediately. A retinal detachment would appear as a shadow or curtain in your vision. If the break occurs in the retina, fluid can leak behind the retina, this pushes the retina forward and eventually causes the retina to detach. An ophthalmologist may recommend treatment to avoid a future detachment. Patients with degenerative myopia that have lattice degeneration or retinal holes are at more risk of having a retina detachment. Thinning of the retina can also cause retinal holes and lattice degeneration (a type of thinning retina).ĝry myopic macular degeneration may affect your ability to see detail, cause straight edges to appear wavy, cause difficulty in reading. ![]() If retinal atrophy occurs at the macula (central vision) it may affect your ability to see detail and colour, this is known as dry myopic macular degeneration. These areas of your vision would appear patchy or blank. The areas of thinned retina may not function properly or at all. Retinal atrophy is the stretching & thinning of the retina this occurs as you become more short sighted and your eyeball becomes longer.Lacquer cracks are fine lines seen on the retina, they are caused by breaks in Bruch’s membrane (Bruch’s membrane is between the retina and choroid, at the back of the eyes), they are usually just monitored and are a warning sign that further complications could occur in the future.ğuch’s spots are the abnormal network of blood vessels in the macula region, which cause damage to the central vision.You would experience a loss of your central vision. Abnormal blood vessels can grow in the damaged areas under the macula, which can then leak fluid & cause scarring. Myopic Macular Degeneration, as the eyeball stretches the layers under the macula (central vision) become stressed and damaged.This will eventually cause distortion to your central vision and can lead to a macula hole or retinal detachment. Where the bulging at the back of the causes the layers of the central vision (macula) to split away from each other. Myopic foveoschisis (myopic traction maculopathy) is caused by Staphylomas.This can lead to loss of vision and sometimes blindness. Staphylomas is when the retina & sclera stretch and thin the back of the eyeball begins to bulge.Pathological myopia may cause the following eye conditions If your central vision is affected – difficulty in seeing fine detail, colour or grey or blank areas.ğrequent changes in your myopia or increases in your prescription. ![]() 9/18/2023 0 Comments Eft gas mask filter![]() ![]() ![]()
9/18/2023 0 Comments Los angeles jdm imports![]() As a result, the dollar appreciated nearly 50 percent against the yen, deutsche mark and other European currencies. By March 1980, the federal funds rate was 20 percent. Federal Reserve Chairman Paul Volcker to dramatically raise interest rates. The stagflation crisis of the 1970s pushed U.S. Unlike today, there was no prohibition on importing a new Euro-Benz.īetter yet, they were cheap. They kept coming because German-spec Mercedes offered horsepower and features not available in American-market Mercedes. I had a good reputation, and they kept coming.” “I was selling high-dollar cars to people paying in advance. “I had so many orders to fill, I couldn’t keep up,” he says. The romance of being a young American expatriate living in Southern Germany was enriched by the lucrative automotive gray market.Īs the decade began, Americans were avidly seeking out German-market Mercedes 500SELs and 280s, and de Board was selling them as fast as he could. The early 1980s were a golden time for Douglas de Board.
9/18/2023 0 Comments Spotify music discovery![]() ![]() Julie McNamara (VP, Head of Global Podcast Studios) will continue to oversee the Spotify Studios organization, with Liliana Kim in charge of Current Content for Spotify Studios alongside Liz Gateley, who will be Head of Development. Furthermore, both studios will greenlight new shows with an increased focus on always-on programming that drives strong, loyal audiences and attracts advertisers. The Ringer will continue to build its unrivaled programming slate across Sports, Culture, and Tech. As part of this next phase, we will be combining Parcast and Gimlet into a renewed Spotify Studios operation that will continue to produce a wide range of high-impact originals, including Stolen, The Journal, Science Vs, Heavyweight, Serial Killers, and Conspiracy Theories. Looking ahead, as a key component of our focus on creators, we remain committed to original programming. The company will support these individuals with generous severance packages, including extended Healthcare coverage and immediate access to outplacement support. Those impacted by this change have already received an invite for a 1:1 conversation today with a member of our HR team, and we are focused on ensuring that each step in this process is taken with the utmost empathy and respect. I want to express my appreciation for everything those leaving have done for podcasting and Spotify. These decisions are not something we take lightly. We know news like this is never easy, especially for those impacted. Unfortunately, this means saying goodbye to close colleagues and friends. As a result, we have made the difficult but necessary decision to make a strategic realignment of our group and reduce our global podcast vertical and other functions by approximately 200 people, or 2% of Spotify’s workforce. However, doing so requires adapting over the past few months, our senior leadership team has worked closely with HR to determine the optimal organization for this next chapter. This fundamental pivot from a more uniform proposition will allow us to support the creator community better. ![]() We are expanding our partnership efforts with leading podcasters from across the globe with a tailored approach optimized for each show and creator. Underpinning this effort is a continued leveling up of our advertising offerings and the introduction of more business models to help more creators make meaningful money from their work. Simultaneously, in collaboration with the podcasting community, we are broadening our analytics capabilities by expanding Spotify For Podcasters, which will help creators maximize their audience on Spotify and beyond. This begins with maximizing consumption from the massive audience we’ve established through format innovation and ensuring that more creators in more places achieve success. Given these learnings and our leadership position, we recently embarked on the next phase of our podcast strategy, which is focused on delivering even more value for creators (and users!). Fortunately, Spotify is not a company that ever sits still. We also know that they appreciate our tools and creator support programs but want more optionality and flexibility in terms of monetization. We know that creators have embraced the global audience on our platform but want improved discovery to help them grow their audience. In addition to fueling a revolution for the medium, this massive group of creators and listeners has also taught us an enormous amount about where podcasting is today and where it is going tomorrow.
9/18/2023 0 Comments Invest 98l spaghetti models 2022![]() ![]() ![]() The next named storm will be called Hermine. Invest 98L spaghetti models for Caribbean 10am Sept 22 Long-range computer forecasts are often plotted in what is called a 'spaghetti model. Here’s what that mean Designation plays a big role in tropical forecasting ORLANDO, Fla. Most models have the goal to be the very best, but each one has a different way of getting to that result. Developing area in Caribbean now designated as Invest 98L. It’s too early to know, however, where the weather system will eventually head or how strong it will be. Each Model Has a Slightly Different Purpose. Long-range computer models take Invest 98L into the Gulf by the end of next week. The National Hurricane Center said the wave has a 70% chance of tropical development over the next two days and a 90% chance over the next five days. Major weather models typically do not do the best job of. We will keep waiting until possibly late. the uncertainty surrounding INVEST 98L will probably continue in a rather significant way over the next few days until the system gets well-established in the central or western Caribbean Sea, Megginson said Thursday. (WWSB) - The quip Waiting on a hurricane is like being stalked by a turtle seems about right for Invest 98L. The system will likely become a tropical depression within the next few days as it is forecast to move west-northwest across the southern Windward Islands by late Wednesday and then toward the central Caribbean Sea later this week. Invest 98L spaghetti models for Caribbean 10am Sept 22 Long-range computer forecasts are often plotted in what is called a 'spaghetti model.' These models show. Published 12:02 pm Thursday, September 22, 2022. ![]() – As two named storms churn in the Atlantic, there’s also a focus on a tropical wave, dubbed Invest 98L, that has the potential to enter the Gulf of Mexico, where conditions are ripe to strengthen a storm.Īs of Wednesday, Invest 98L was producing shower and thunderstorm activity a few hundred miles east of the southern Windward Islands. ![]() ![]() ![]() Of my favorite party memories are from the end of a night, when a few close friends stay behind and help pick up glasses, emptyĪshtrays, and put pillows back on sofas. design partner cards (Premium Card) and/or you add an optional design element designated as premium to any card, free or premium (Premium Designs). A card is premium and paid-for if the card itself is designated premium on the site e.g. ITSBEEN A Wooo00000 TIMESINCE IVE SEN YOU. Paperless Post offers free and premium card and invitation designs (referred to here as cards). ![]() Seated dinnersīring out the worst in even the closest of friends. Unless its a super formal event, or youre trying to set someone up. DO throw your own party My mom always said, If you want something done right, do it yourself. ![]() Get Started Invitations Get Started Effortless Online Invitations and RSVP Tracking Deliver elegant online invitations directly to all of your friends and family with Greenvelope. Whether its golf, bowling, gymnastics, swimming, tennis. PARTY PEOPLE Do this, not that WITH DEREK BLASBERG The bestselling author, Paperless Post design partner, and quintessential manĪbout town shares his advice for fting a birthday like the grownup you are. Create & Send Beautiful Online Invitations for Any Occasion Make it your own with photos, music, & more. Whatever the sport, Paperless Post has an online kids birthday party invitation to match your theme. ![]() 9/18/2023 0 Comments Quadcopter drone maker![]() ![]() Russian forces, in particular separatist units, are also receiving consumer drones for tactical reconnaissance – again from donations rather than the usual military acquisition process. (Even DJI drones can drop small grenades, and Ukrainian volunteers have posted at least one video claiming to show this approach). Ukrainian Aerorozvidka reportedly use consumer quadcopters with thermal imagers to find Russian forces at night, before bombing them with anti-tank grenades from larger, locally-made R18 octocopters or calling in artillery fire with Starlink satellite communications. Vast numbers of drone videos show Ukrainian artillery carrying out highly accurate strikes on Russian forces. Although Ukraine has some military-grade spotter drones, including locally made Fury, Spectator and Stork models, there are not enough to go around, but there are thousands of DJI and other quadcopters. Ukrainian military drone operator Oleh Sobchenko, interviewed by Euromaidan Press, confirms that volunteer drone operators with consumer drones are doing much of the target location and fire direction for Ukrainian artillery. This control means that mortars can even destroy tanks with direct hits, rare in previous conflicts. The mortar, a simple, low-tech device for lobbing bombs in the general direction of enemy positions becomes vastly more lethal when the drone operator can find the enemy, then direct mortar fire on to their location, reporting on the fall of every shot so it can be adjusted on the target. One video shows a Ukrainian mortar team equipped with a quadcopter. ![]() ![]() Aerial reconnaissance was previously confined to higher levels of command and only gradually filtered down the chain now every squad leader can have a folding Mavic Mini 2 small enough to fit into a cargo pocket but able to send back HD video from three miles away for half an hour. At its simplest, a small drone is an eye in the sky, giving what every commander has wanted for centuries: a view of what is happening on the other side of the hill, or in the middle of the forest, or just down the next street, without having to send troops. ![]() |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |